In this work, a commercial Supercritical Fluid Chromatography (SFC) instrument has been in-house modified through a series of technical adjustments in order to reduce its extra-column variance by more than 230% with respect to the original configuration. State-of-the-art efficiencies of ultra-high performance Whelk-O1 chiral columns have been obtained with the optimized version of the instrument.
Green tea (GT) is made from the leaves of Camellia Sinensis and is the most popular beverage in Asia. The catechins content is associated to the biological value of tea and it could be used as potential chemical indicator of the geographical origin. A capillary electrophoresis method was developed for the characterization of GT samples by evaluation of the quantitative profile of catechins.
The thickness and composition determination of thin films it is an important task in many industrial environments. The XRF is commonly employed but it needs the exact knowledge of the composition and disposition of the all layers.
We explored the advantages of EDX for analysis of both the thickness, exploiting a calibration curve built with Monte Carlo simulated standards, and the composition.
As part of the Regional POR CREO GADGET - ERDF 2014-2020 Call No.2 project, sequencing studies of precious metal alloys are planned in order to increase their resistance to corrosion and wear. These studies provide for the knowledge and use of the main metal Electrodeposition techniques and of the main electrochemical techniques for the development of galvanic baths.
Nature-derived hydrogels are a promising class of scaffolds for tissue engineering applications. This work describes the use of Manuka honey for preparing gellan gum-based composites with antibacterial properties and superior compressive Young’s modulus. The hydrogel has been characterized by different analytical techniques and its mechanical and biological properties have been also evaluated.
The purification of a non-mAb protein has been performed my means of split-intein mediated affinity chromatography. The purification of the target protein from the crude lysate occurs through the great affinity between N- and C-intein tags, fused to the resin and the protein, respectively. In this work, such a stationary phase has been characterized and the purification protocol optimized.
Paper is showed as a reactor. To demonstrate this, Prussian Blue Nanoparticles (PBNPs), were synthesized on filter paper without external inputs. The functional paper, coupled to screen-printing, is successfully applied to detect blood glucose. This device demonstrated a good correlation with commercial glucose strips. This approach is also extendible to other (bio)systems.
Chromatography (semi-)preparative-scale stereoresolution of pharmaceutically relevant compounds performed with low- and high-molecular weight chiral selectors.
Selection of exemplary cases in which the “racemic approach” represented the elective choice, with respect to the “chiral approach”, at the discovery stage when preliminary biological tests with single stereoisomers have to be carried out.
Thin films made of various materials are used in many scientific, technological and industrial environments. They are deposited through a variety of physical, chemical and electrochemical techniques. We present a new methods to measure the thickness, the colour, the morphological and compositional of the film.
The multivariate interpretation of the urinary steroidal profile (USP), consisting in a combination of PLS-DA classification with regression and likelihood ratio, is proposed as new screening tool for the prostatic malignancy. The USP is detected by GC-MS, limited in costs and widely available. This procedure provides better performances with respect to the current screening based on PSA and DRE.
A new approach, SO-CovSel, given by the combination of a multi-bock regression method,called SO-PLS, with a variable selection method, Covariance Selection (CovSel), has been proposed. SO-CovSel gave predictions comparable to SO-PLS’; when applied in a classification framework (by combination with discriminant analysis) SO-CovSel-LDA provided equal or better results than SO-PLS-LDA.
The aim of this study is to investigate how different water activities affect rice germ shelf life. In fact, this matrix (a by-product of rice milling process) could be interesting for human nutrition but, for its composition characterized by unsaturated fatty acids, it undergoes rancidity during storage.
(NON CONSENTE DI CARICARE L'INTERO TESTO CHE è STATO ALLEGATO)
In this study, a massive sampling of wine samples have been conducted to develop multivariate models for the identification of wines produced in the Chianti area. The element content profile based on ICP-MS was determined on 639 red wine samples and thus used as input for the subsequent calibration of multivariate classification models.
376 hazelnuts have been unshelled and analyzed by NIR spectroscopy. Then, SIMCA and PLS-DA models, aimed to distinguish Nocciola Romana PDO samples from common hazelnuts have been created. SIMCA analysis gave the best results: 4 test objects (over 49) belonging to Class PDO are rejected from the model and 1 object (over 41) from Class common was accepted.
The study proposes the use of novel surfaces for the desorption electrospray ionization-high resolution mass spectrometry (DESI-HRMS) determination of new psychoactive substances in oral fluids. A microextraction by packed sorbent approach was optimized for the extraction of the investigated analytes from saliva samples. Finally the method was validated and applied for the analysis of real samples
The present study reports the development of a new method of extraction of volatile fatty acids (VFA) from digestate samples of anaerobic digestion with dimethyl carbonate (DMC), followed by GC-MS analysis. The use of solid-phase microextraction (SPME) was investigated for the first time as fast method for the analysis of volatile methyl siloxanes (VMS) in raw biogas and upgraded biomethane.
Lanthanide complexes with ligands such as DOTA and its derivatives are used as contrast agents for Magnetic Resonance Imaging (MRI). The use of complex ions originates in the toxicity of most of the paramagnetic metals. To prevent their bond with molecules inside the body, and therefore to reduce their toxicity, these metal ions are masked with ligands able to chelate.
Understanding dynamic interaction mechanisms between polymeric scaffolds and ECM simplified proteins represent an important step for the improved efficacy in tissue regeneration. Here MS is applied to study the interactions between a fibronectin fragment and a specific aptamer on chitosan-based films. The effect of the functionalization on the colonization by osteoblastic cells is provided.
The knowledge of steroidal profile alterations, related to physical training, is important to avoid the occurrence of false positive results in anti-doping screening. The urinary steroidal profiles of two endurance athletes were time monitored. Then, a “reference” PCA model was built and tested using the post-training samples. The physical effort proved to be a bias factor in the USP evaluation.
The present research aimed at modelling the effect of the variables time and temperature as a function of the levels of fatty acids in liver of Antarctic fish Trematomus bernacchii.
The proposed models were in agreement with reported studies on polar and temperate fish, indicating possibly similar adaptation mechanisms for teleost to cope with global warming.
Microplastics (MPs) have been identified as contaminants of emerging concern in aquatic environments. This study aims to quantify and characterize MP pollution in sediment, snow and ice-core samples of Vesijärvi and Pikku Vesijärvi Lakes (Finland). Lake Vesijärvi is a big attraction for tourists and the winter activities taking place on the frozen surface could represent remarkable sources of MPs.
We aimed to engineer nucleic acid nanostructures able to process information through programmable molecular motion and assembly. We set out to design nucleic acid-based actuators that can generate binding-responsive measurable outputs. We focused on antibodies and transcription factors as biomolecular targets and proposed innovative technologies for their detection in solution and in vitro.
Tissue engineering is a field of regenerative medicine that relies on supporting material and cells to develop a biological substitute. Here, the relation within the physicochemical features of Chitosan and the features of a final 3D printed scaffolds has been investigated. In-vitro studies on human skin cell growth are reported to demonstrate the cytocompatibility of the 3D printed scaffolds.